Elucidation of the mechanisms for antigen uptake by gill eputhelial antigen sampling cells

2022 Fiscal Year Final Research Report

• Project/Area Number: 20H03061
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 40030:Aquatic bioproduction science-related
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: Kato Goshi 東京海洋大学, 学術研究院, 准教授 (50624219)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 魚類免疫学

Outline of Final Research Achievements

Previously, we showed that gill epithelial antigen sampling (GAS) cells take up inactivated Vibrio anguillarum and Aeromonas salmonicida subsp. salmonicida (A.s.s.) after immersion vaccination, while no uptake of polystyrene beads were observed. In this study, we investigated what kind of bacteria are taken up by GAS cells in the gill epithelium. GAS cells harboring inactivated bacteria were found in A.s.s.-vaccinated group, in V. anguillarum-vaccinated group, and in Y. ruckeri-vaccinated group. In contrast, a few, or no GAS cells positive for inactivated cells of F. psychrophilum, S. iniae, and E. coli were observed after Ex vivo immersion. In this way, GAS cells uptake the specific type of bacteria during immersion vaccination, suggesting that the cells are responsible for the mechanisms underlying efficacy of immersion vaccines.

Free Research Field

魚類免疫学

Academic Significance and Societal Importance of the Research Achievements

本研究によりニジマスの鰓で浸漬ワクチンを取り込むGAS細胞が、特定の細菌主の身を取り込むことが明らかになった。このことは浸漬ワクチンが有効性を示す感染症と、ほとんど効果を示さない感染症があることの科学的裏付けになると考えられる。今回の研究では宿主側のレセプター分子および細菌側のリガンド分子の同定には至らなかったものの、今後引き続きこれら分子の同定を行うことで、魚類の新しい浸漬ワクチンデリバリーシステムの構築を行っていきたい。