Elucidation of interaction mechanisms improving ligninolytic properties in white rot fugal-bacterial consortia

2022 Fiscal Year Final Research Report

• Project/Area Number: 20H03114
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 41050:Environmental agriculture-related
• Research Institution: Shizuoka University
• Principal Investigator: Mori Toshio 静岡大学, 農学部, 准教授 (80536516)
• Co-Investigator(Kenkyū-buntansha): 鈴木 智大 宇都宮大学, バイオサイエンス教育研究センター, 准教授 (10649601) ; 河岸 洋和 静岡大学, グリーン科学技術研究所, 教授 (70183283)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 白色腐朽菌 / 複合微生物系 / 微生物間相互作用 / リグニン分解 / 木材腐朽

Outline of Final Research Achievements

Microbial consortia consisting of white-rot fungus Phanerochaete sordida and natural occurring bacteria were created, and a subcultivation method maintaining excellent ligninolytic properties was developed. Based on prediction of metagenomic functional abundances was conducted from the bacterial community structure of the consortia, it was predicted that oxidative stress substances like naphthoquinone derivatives would inhibit fungal growth, thereby reducing polysaccharide consumption, and inducing ligninolytic enzymes, then the consortia would exhibit highly selective lignin degradation activity. Furthermore, redundancy analysis using ligninolytic activity and microbial community structure of the consortia was perfomed to identify bacteria that induce ligninolytic enzyme activity, resulting several candidate bacteria were successfully obtained.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

白色腐朽菌はリグニンを分解し、しばしばセルロース等多糖成分に富んだ腐朽残渣を生じるため、低環境負荷型脱リグニンツールとしてバイオマス利用分野への応用が期待されているが、その活性は実用レベルではない。白色腐朽菌の木材腐朽特性を改善・制御する細菌との相互作用機構を明らかにしていくことで、試薬や遺伝子組換えに頼らない細菌との相互作用を利用した白色腐朽菌機能の制御技術が提案可能となる。本研究成果は、資源循環型社会構築に寄与しうる、新たな微生物活用技術開発に向けた第一歩となる。