2022 Fiscal Year Final Research Report
Establishment of basic technology for poultry reproductive engineering by developing germ cell-free chickens
Project/Area Number |
20H03135
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 42010:Animal production science-related
|
Research Institution | National Institute of Advanced Industrial Science and Technology |
Principal Investigator |
Oishi Isao 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (50314472)
|
Co-Investigator(Kenkyū-buntansha) |
迎 武紘 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究員 (40803309)
|
Project Period (FY) |
2020-04-01 – 2023-03-31
|
Keywords | 始原生殖細胞 / ニワトリ / 遺伝子ノックイン / 細胞死誘導 / 生殖工学 |
Outline of Final Research Achievements |
We aim to create and utilize germline-free chickens, an important part of chicken reproductive engineering technology. By using germline-free chickens as recipients and transplanting frozen germline cells or genetically engineered cells, we can create germline chimeric chickens with a high donor contribution rate, thereby conserving genetic resources and increasing efficiency in producing genetically engineered individuals. We have attempted to induce germline-specific cell death by creating lines that inducibly express specific genes. Some lines were successful and fertility was confirmed, but other lines still need to be improved. While working to improve the germ cell death induction system, we aim to conserve the genetic resources of chickens and realize an efficient technology for producing genetic modifications.
|
Free Research Field |
分子生物学
|
Academic Significance and Societal Importance of the Research Achievements |
ニワトリは産業動物として重要であり、品種改良や遺伝育種も進んでいます。一方、鳥インフルエンザの蔓延や希少品種飼育者の高齢化などで折角の遺伝資源が失われてしまう危機も強まっています。このような状況で生殖工学を用いたニワトリ遺伝資源の保全は重要であり、生殖細胞を持たないニワトリ系統作出は大きな進歩となります。本研究で生殖細胞特異的に遺伝子を制御できる基盤が構築されました。これは生殖細胞を持たないニワトリ開発に向けた足がかりであり、得られた研究資源や知見もとに生殖工学技術に資する系統開発を進めることができます。
|