Distribution analysis of S1P on tissue sections by high resolution MS imaging

2022 Fiscal Year Final Research Report

• Project/Area Number: 20H03374
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related
• Research Institution: Teikyo University (2021-2022); Tohoku University (2020)
• Principal Investigator: Saigusa Daisuke 帝京大学, 薬学部, 准教授 (90545237)
• Co-Investigator(Kenkyū-buntansha): 可野 邦行 東京大学, 大学院薬学系研究科(薬学部), 助教 (50636404) ; 松本 洋太郎 東北大学, 薬学研究科, 講師 (90420041)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: S1P / MALDI / MSイメージング / 質量分析計 / スフィンゴ脂質

Outline of Final Research Achievements

Sphingosine monophosphate (S1P)　is a bioactive lipid and has been implicated in the pathogenesis of immunological diseases, cancer, and atherosclerosis. S1P and its related molecules expressed in their signaling pathway are considered potential drug targets. However, it has been difficult to visualize the tissue distribution of S1P using conventional analytical methods. In this study, we developed a S1P-specific detection method and a highly sensitive and high-resolution tissue section by MS imaging. We constructed a library of Phostag derivatization reagents by synthesizing novel Phostag derivatization reagents, and succeeded in highly sensitive detection of S1P.

Free Research Field

臨床分析化学

Academic Significance and Societal Importance of the Research Achievements

我々は、S1Pを高感度に検出できる新規Phostag試薬の合成に成功した。本研究では、Phostag誘導体化試薬を複数種合成したことにより、Phostagライブラリーを構築したが、この試薬類は様々な化合物に対して応用できる可能性が考えられるため、学術的な意義が高い。また、S1P関連疾患に対する分子メカニズム解析にも有用であり、疾患解明に貢献するため社会的意義が高いと考えられる。本研究により新たな創薬に役立つことが期待される。