2022 Fiscal Year Final Research Report
Targeting sarcoma stem cells using immunological memory T cells.
| Project/Area Number |
20H03807
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 56020:Orthopedics-related
|
|---|
| Research Institution | Sapporo Medical University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2020-04-01 – 2023-03-31
|
| Keywords | CAR-T / TCR-T / HLA/ペプチド複合体 / がん抗原 |
|---|
| Outline of Final Research Achievements |
We developed a new CAR using B10 scFv clone directed to HLA-A24/cancer stem cell antigen DNAJB8-derived peptide complex (B10 CAR). B10 CAR-T cells showed anti-tumor effects against HLA-A24+/DNAJB8+ cancer and sarcoma cell lines in vitro and in vivo. Moreover, we developed a new TCR using TCR of CTL clone directed to osteosarcoma antigen PBF-derived peptide (PBF TCR). PBF TCR-T cells showed anti-tumor effects against HLA-A24+/PBF+ sarcoma cell lines in vitro and in vivo.
|
| Free Research Field |
がん免疫，骨軟部腫瘍
|
| Academic Significance and Societal Importance of the Research Achievements |
骨肉腫をはじめとする高い悪性形質を示す肉腫は時に若年者に発症し，肺転移例や化学療法不応例の予後は不良である．本研究成果は，末期肉腫患者に対する遺伝子改変T細胞療法の開発に有用な可能性がある．
|
