2023 Fiscal Year Final Research Report
Identification of novel dental pulp stem cells and analysis of their regulatory mechanisms of differentiation
| Project/Area Number |
20H03853
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 57010:Oral biological science-related
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
東 俊文 東京歯科大学, 歯学部, 教授 (00222612)
小林 泰浩 松本歯科大学, 総合歯科医学研究所, 教授 (20264252)
細矢 明宏 北海道医療大学, 歯学部, 教授 (70350824)
西田 大輔 東京歯科大学, 歯学部, PF (00843608)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 象牙芽細胞 / 修復象牙質 / 歯髄幹細胞 / 細胞稠密層 / 細胞系譜解析 / 細胞の枯渇実験 / PTH |
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| Outline of Final Research Achievements |
We have found that reparative dentin formation is induced by odontoblast death, and the expression of parathyroid hormone receptor (PTHR) in pulp tissue is upregulated during this process. Based on the previous above findings, the following findings were revealed in our study. (1) In response to odontoblast death, proliferative activity of Nestin(Nes)-positive and -negative dental pulp cells localized in the cell-rich zone was increased and they differentiated into type I collagen(Col1)-positive odontoblast-like cells. (2) Administration of teriparatide, a biologically active N-terminal 1-34 peptide of the human PTH, increased the formation of reparative dentin following osteoblast depletion. (3) Administration of teriparatide tended to increase the formation of reparative dentin induced by physical defects in dentin. (4) Nes-positive cells in the cell-rich zone were found to be quiescent and long-lived.
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| Free Research Field |
骨代謝
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| Academic Significance and Societal Importance of the Research Achievements |
口腔は、咀嚼機能を介して生命維持の根幹を司る重要な器官であり、この咀嚼機能の発現には、正常な歯牙硬組織の維持が必須である。したがって、臨床の現場では、象牙質欠損をより速やかに補填する新規治療法の開発が求められているが、その硬組織修復メカニズムは未だ良くわかっていない。本研究成果により、象牙芽細胞の細胞死が細胞稠密層に局在する歯髄細胞を介して修復象牙質形成に寄与することが明らかになった。今後は、以上の研究成果をさらに発展させ、人為的な修復象牙質形成の誘導を可能とする新規硬組織再生治療法の開発を目指した基盤研究の創生に繋がることが期待される。
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