2022 Fiscal Year Final Research Report
Development of cell separation technology using a quadrupole capillary dielectrophoretic device
Project/Area Number |
20K04276
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 19010:Fluid engineering-related
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Research Institution | 防衛大学校(総合教育学群、人文社会科学群、応用科学群、電気情報学群及びシステム工学群) |
Principal Investigator |
Tada Shigeru 防衛大学校(総合教育学群、人文社会科学群、応用科学群、電気情報学群及びシステム工学群), 応用科学群, 教授 (70251650)
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Co-Investigator(Kenkyū-buntansha) |
松元 藤彦 防衛大学校(総合教育学群、人文社会科学群、応用科学群、電気情報学群及びシステム工学群), 応用科学群, 教授 (10531767)
江口 正徳 呉工業高等専門学校, 電気情報工学分野, 准教授 (60613594)
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | 細胞分離 / 誘電泳動 / 4重極電極 / キャピラリー |
Outline of Final Research Achievements |
Cell separation technology based on the principle of dielectrophoresis has been attracting attention as a technology for separating cells at high speed and with high accuracy. In this study, we proposed a quadrupole capillary dielectrophoresis device as a new cell separation device. Numerical simulations of the electric field were performed to optimize the device design and to evaluate its operating characteristics, and at the same time, the device specifications were determined. To evaluate the cell separation efficiency of the proposed device, the device was fabricated according to the design, and a mixed solution of live and dead human mammary epithelial cells was poured into the cell sample, and experiments were conducted to separate live and dead cells. As a result, it was observed that cells were properly separated when the number of cells was small, but when the number of cells increased, the separation efficiency was not as expected.
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Free Research Field |
生体流体工学
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Academic Significance and Societal Importance of the Research Achievements |
提案デバイスが持つ①誘電泳動力がガラス管断面内全域の細胞に対して作用するため、高効率の分離が期待できる。②電極との接触による細胞の損傷や溶液の電気分解、電圧降下、電極の劣化等のリスクがない。といった特徴は、従来型デバイスが抱える幾つかの問題点を発熱の問題を除き、解決出来る。本研究成果については、細胞分離率の向上など、今後解決されるべき課題点はあるが、新しい概念による誘電泳動型細胞分離デバイスの有効性が示され、誘電泳動技術を応用した臨床検査技術の発展に大きく貢献する。また誘電泳動デバイスの研究と臨床応用に大きな質的転換をもたらすことが期待出来る。
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