2022 Fiscal Year Final Research Report
Acceleration method of microbially induced calcite precipitation for prolonging service life of landfill site facilities
| Project/Area Number |
20K05202
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 27010:Transport phenomena and unit operations-related
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| Research Institution | Fukushima National College of Technology |
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Principal Investigator |
Kurumada Kenichi 福島工業高等専門学校, 化学・バイオ工学科, 教授 (80273473)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 不溶性炭酸塩晶析 / 微生物代謝起因 / ウレアーゼ活性 / 一般自然環境自生型 / 野外環境 / 増殖ブースター / バイオマス素材 / 選択的増殖工程 |
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| Outline of Final Research Achievements |
Microbaially induced carbonate precipitation was shown effective for converting dissolved calcium ion to insoluble carbonate, which could be applied to enhancing the mechanical strength of soil due to the formation of the inter-particle bridging. Urease-active microbes are indispensable for generating carbon dioxide which resulting in the crystallization of the insoluble carbonate. Although in-lab experiments normally use purchased urease-active microbes, large-scale field tests never allows us to adopt them due to the expensive price of them and lack of the evidence that artificially induced microbes selectively contribute to the carbonate crystallization. To avoid the ambiguity of the efficacy of the artificially introduced microbes for forming the carbonate, high-biosafety microbes were propagated at by supplying dilute urea aqueous solution in advance. The preliminary propagation of the urease-active microbes from conglomerates of indigenous microbes in common natural environment.
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| Free Research Field |
化学工学，工業単位操作
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| Academic Significance and Societal Importance of the Research Achievements |
①輸入等に依存する炭酸塩発生能具有型外来種導入ではなく自生種の利用方法を見い出し，微生物代謝由来の不溶性安定炭酸塩の晶析の応用可能性を拡大した。手法根幹は有用ウレアーゼ活性型細菌群を分率増大させるバイオマス材の介在に拠る二酸化炭素発生の人為的誘発にある。②上記ウレアーゼ活性促進に遍在バイオマス類が活用可能である。廃バイオマス植物枯死体をウレアーゼ活性のブースター菌源として試用し系中細菌群の遺伝子解析を実施したところバイオセーフティ上の問題はなかった。それら細菌は全て遍在種であり，かつ適切な条件下において最も優れた炭酸塩晶析誘引能があるパステウリと比較して遜色がない誘引能を具える安全種群だった。
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