2022 Fiscal Year Final Research Report
Development of a device for amino acid analysis via an encompassing analysis of multiple disease states
| Project/Area Number |
20K05237
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 27040:Biofunction and bioprocess engineering-related
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| Research Institution | Hiroshima City University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | アミノ酸 / アミノアシルtRNA合成酵素 / アミノグラム / 生体計測 / バイオセンサー / ペーパーマイクロ流路デバイス |
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| Outline of Final Research Achievements |
Free amino acid contents in blood serum serve as an indicator of a disease state, such as cancers, hepatic diseases, and diabetes; hence, they are considered useful in clinical diagnostics. A one-step analysis method was developed for amino acids, valine, leucine, and isoleucine, using a microfluidic paper-based analytical device fabricated from chromatography filtration paper and laminate films. Aminoacyl-tRNA synthetase was used to detect each amino acid. The obtained laminated paper-based analytical device (LPAD) contained four enzymatic reaction areas. Colorimetric detection was performed based on the molybdenum blue reaction.
The method provided a selective quantification at the ranges of several micromolar to 100 uM for valine, leucine, and isoleucine, respectively. LPAD fabrication was considerably simple, and the subsequent detection process was easy and required a short period of time (within 15 min).
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| Free Research Field |
生物機能工学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では生体の栄養状態などを評価するのに有用なアミノ酸であるバリン、ロイシン、イソロイシンをそれぞれ計測可能な、紙で評価を行うペーパーデバイスの開発に成功した。計測可能な各アミノ酸の濃度範囲について、血液中のアミノ酸濃度の範囲を満たすこともできた。血液中の各アミノ酸濃度を個別に計測することによって、がんや肝臓病、糖尿病などの各種病態の診断が可能であることが知られており、本研究で得られた方法を他のアミノ酸にも適用することで、将来的には様々な病態が家庭において簡易に計測できる可能性がある。
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