2023 Fiscal Year Final Research Report
Development of a method for immobilizing functional molecules on solid-liquid interfaces using self-assembled monolayers
| Project/Area Number |
20K05251
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 28010:Nanometer-scale chemistry-related
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Nakano Miki 国立研究開発法人産業技術総合研究所, エレクトロニクス・製造領域, 主任研究員 (20415722)
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| Co-Investigator(Kenkyū-buntansha) |
松田 直樹 国立研究開発法人産業技術総合研究所, エレクトロニクス・製造領域, 上級主任研究員 (10344219)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | 固液界面 / 機能性分子 / 吸着 / 自己組織化膜 / 脱離 / 吸収スペクトル |
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| Outline of Final Research Achievements |
The largest technical issue to the development of devices utilizing interfaces is that it is impossible to confirm the immobilized ratio from the adsorbed molecules and if these immobilized molecules maintain their function or not. To control the interfacial phenomena, it is of much importance to narrow down which reagent for making self-assembled monolayer (SAM) should be used. In this study, in-situ observations with slab optical waveguide spectroscopy were established as a method to evaluate the fixation ratio of adsorbed molecules and their functional maintenance. Cytochrome c (Cytc) fixation ratio was significantly increased, and redox function was maintained when thin glass sheets with SAM of hydrophobic silane and hydrophilic phosphonic acid compounds were used. Furthermore, when the adsorption time of Cytc increased prior to the evaluation of the fixation ratio, the immobilized ratio increased, suggesting that the conformational change of Cytc induced because of the adsorption.
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| Free Research Field |
表面科学
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| Academic Significance and Societal Importance of the Research Achievements |
最先端技術でも固液界面におけるその場観察は対象物質が極微少量のため吸着量の変化や分子の機能維持判定は非常に困難である。そこで本研究では吸着の絶対量変化ではなく、吸着量の減少割合を可視領域の吸収スペクトル減少から計測した点で独創的な研究内容であり学術的意義が大きい。これらの成果が今後のデバイス開発に積極的に用いられることを想定すると、社会的意義も大きい。
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