2022 Fiscal Year Final Research Report
Development of comparable intracellular stability evaluation method for nanomaterials using intracellular delivery nanocarriers
Project/Area Number |
20K05284
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 28040:Nanobioscience-related
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Research Institution | Health Sciences University of Hokkaido |
Principal Investigator |
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | 細胞内送達ナノキャリア / ナノ材料の細胞内安定性 / 磁性ナノ粒子 / ナノバイオ |
Outline of Final Research Achievements |
The aims of this study were to develop nanocarriers that deliver various nanomaterials into cells and evaluate their intracellular stability and to search for suitable ligands for nanomaterials with high intracellular stability. First, in the development of the nanocarriers, a silica shell was formed on the surface of magnetic nanoparticles, the base material of the nanocarriers, to enhance the intracellular stability of the nanocarriers themselves. Next, to prevent aggregation of the nanoparticles in a process of molecular modification, we searched for suitable dispersant that improve the dispersion stability of the nanoparticles. Finally, in the search for ligands, we first selected ligands in terms of dispersion stability of nanoparticles modified it and reactivity with amines, and for the superior ones, we conducted dispersion stability tests under the condition similar to the intracellular environment to narrow down the candidate compounds.
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Free Research Field |
分析化学
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Academic Significance and Societal Importance of the Research Achievements |
本研究で目標とした細胞内送達ナノキャリアは期間内の完成には至らなかったが、開発過程で得られたナノ粒子の分散安定化に関する知見は、多くのナノ材料開発に役立つものと期待される。昨今、ナノ材料をコアとする技術・製品が次々と社会実装されており、これまで以上にナノ材料が身近になる社会にとって、生体内あるいは細胞内におけるナノ材料の安定性(安全性)の評価は重要になるものと考えられる。
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