• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2022 Fiscal Year Final Research Report

Elucidation of D-amino acid-induced protein expression by analyses of gene expression and amino acid dynamics

Research Project

  • PDF
Project/Area Number 20K05729
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 37020:Chemistry and chemical methodology of biomolecules-related
Research InstitutionThe University of Tokushima

Principal Investigator

KAWAKAMI Ryushi  徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 准教授 (90380120)

Project Period (FY) 2020-04-01 – 2023-03-31
Keywords遺伝子発現 / D-アミノ酸 / 転写因子 / 超好熱アーキア
Outline of Final Research Achievements

In this study, we attempted to elucidate the expression mechanism of amino acid racemase (BAR) in the hyperthermophilic archaea Pyrococcus horikoshii OT3 using gene expression analysis, EMSA analysis, and X-ray crystallographic analysis. It was found that the gene expression was controlled by transcription factor protein interacting with D-amino acid. This is the first observation that D-amino acids function as physiologically active substances in archaea.

Free Research Field

微生物酵素学

Academic Significance and Societal Importance of the Research Achievements

今回の研究で、超好熱アーキアがD-アミノ酸を生理機能物質として利用していることを初めて明らかにした。D-アミノ酸の生体内における生理機能はヒトなどの高等生物やバクテリアにおいても知られており、アーキアの中でも共通祖先に近いとされる超好熱アーキアでのD-アミノ酸機能の発見は進化の観点からも興味深い。今後のD-アミノ酸研究の新たな基盤づくりに貢献できたと考えている。

URL: 

Published: 2024-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi