2022 Fiscal Year Final Research Report
Analysis of the molecular mechanisms for sporangium dehiscence in Actinoplanes missouriensis
Project/Area Number |
20K05781
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 38020:Applied microbiology-related
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Research Institution | The University of Tokyo |
Principal Investigator |
Tezuka Takeaki 東京大学, 大学院農学生命科学研究科(農学部), 助教 (80646414)
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | 胞子 / 胞子嚢 / 休眠 / Clp複合体 / 希少放線菌 / peptidase / ATPase |
Outline of Final Research Achievements |
The object of this research project is to clarify the molecular mechanisms for the transfer from vegetative mycelia to spores and for the activation of the dormant cells to germinate in an actinomycete Actinoplanes missouriensis. The specific targets of the research are follows: (i) regulatory mechanism for the transcription of clpX, which encodes an ATPase subunit of the chaperonin-linked protease (Clp) complex essential for the formation and dehiscence of sporangia, (ii) Identification of the peptidase subunits in complex with ClpX during sporangium formation and dehiscence, and (iii) identification of the target proteins of degradation by the Clp complex during sporangium dehiscence.
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Free Research Field |
応用微生物学
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Academic Significance and Societal Importance of the Research Achievements |
原核生物の中には休眠耐久細胞として胞子を形成するものが知られており、これは貧栄養な環境が大半を占めると予想される自然界において重要な生存戦略である。本研究では、希少放線菌Actinoplanes missouriensisを材料として、胞子が包まれた袋状の構造物である胞子嚢が形成される過程、および胞子嚢が外部環境の変化を感知して開裂し、胞子を放出する過程を制御する分子機構の解明を目的とし、胞子嚢の形成と開裂に必須であるChaperonin-linked protease (Clp) 複合体の機能解析を行った。
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