DNA repair mechanism by condensed nucleoid-dependent end joining

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K05813
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38020:Applied microbiology-related
• Research Institution: Toyo University
• Principal Investigator: Narumi Issay 東洋大学, 生命科学部, 教授 (90343920)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: DNA修復 / 放射線抵抗性細菌 / DNA損傷誘導性 / ゲノム2本鎖切断修復

Outline of Final Research Achievements

The DdrAP protein, which is structurally similar to the DNA damage-inducing protein DdrA, is highly conserved in Deinococcus sp. The gene encoding DdrAP forms an operon with the dr0042 gene encoding the DR0042 protein located immediately below it. DR0042 protein forms a complex with DdrAP protein and regulates the interaction of DdrAP protein with DNA repair-related proteins such as PprA protein involved in condensed nucleoid dependent end joining (CNDEJ). Furthermore, it is suggested that DdrAP protein dissociates from DR0042 protein and forms a complex with DdrA protein after DNA repair is completed, thereby promoting the rapid inactivation of DdrA protein, which is an impediment to replication.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

放射線抵抗性細菌に特有のDNA修復機構である凝縮核様体依存性末端結合（CNDEJ）の構成要素としてこれまでに、PprAタンパク質、DNAジャイレース、DNAリガーゼが明らかになっている。本研究によって、DR0042タンパク質がDdrAタンパク質及びDdrAPタンパク質と相互作用することが示唆され、CNDEJの構成要素として放射線抵抗性細菌が持つDNA損傷誘導性のDNA修復機構の一端を担うことが明らかになった。機構の解明は、新たな遺伝子工学試薬の開発にも繋がり、生命科学の新たな展開に貢献すると考えられる。