Development of high-throughput analysis of plasmalogens using enzyme

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K05822
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38030:Applied biochemistry-related
• Research Institution: Fukushima University
• Principal Investigator: Sugimori Daisuke 福島大学, 共生システム理工学類, 教授 (40272695)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: ホスホリパーゼD / プラズマローゲン

Outline of Final Research Achievements

Development of simple and rapid assay of choline-type and ethanolamine-type plasmalogens, which are attracting attention as biomarkers for early detection of Alzheimer's dementia and arteriosclerosis, has attracted attention. We have found two types of enzymes, and studied their recombinant production. Although there are still issues with the production, we have confirmed that production using E. coli was possible and that quantification of the plasmalogens was possible.

Free Research Field

生物工学

Academic Significance and Societal Importance of the Research Achievements

本研究では、コリン型およびエタノールアミン型プラズマローゲンに選択的に作用する酵素を見出した。この特徴は、新規なものであり、その特徴を発揮するメカニズムが今後解明されれば、これまで未解明であった分子認識機構の理解につながる点で重要な発見といえる。本酵素を応用し、将来血液1滴あれば簡単かつ短時間でアルツハイマー型認知症や動脈硬化症の早期発見が可能になることが期待できる。