Analysis of receptor activation in Brassica self-incompatibility

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K05824
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38030:Applied biochemistry-related
• Research Institution: The University of Tokyo
• Principal Investigator: Murase Kohji 東京大学, 大学院農学生命科学研究科(農学部), 特任准教授 (50467693)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 自家不和合性

Outline of Final Research Achievements

Self-incompatibility responses in Brassica are triggered by the haplotype specific interaction of pistil receptor kinase SRK and pollen ligand SP11. In this study, we tried crystallization of SRK kinase domain for uncovering the mechanism of SRK signal transduction. We determined SRK region which could be highly and stably expressed in bacteria cells. The SRK kinase proteins were expressed in large scale, purified three step chromatography, and crystal screened. Diffraction data were collected in SPring-8 and best data showed 4.3 angstrom resolution.

Free Research Field

植物科学

Academic Significance and Societal Importance of the Research Achievements

アブラナ科植物の自家不和合性は雑種強勢を利用したF1ハイブリッド種子の生産に利用されており、自家不和合性を人為的に操作できる技術の開発が望まれている。SRKキナーゼドメインを阻害する薬剤はいくつか報告されているが、SRKに特異的な阻害剤を開発するためにはSRKキナーゼドメインの構造情報が重要である。本研究ではSRKキナーゼドメインの結晶化に成功し、構造決定に向けて一歩前進した。