Natural product discovery by a combination of genome mining and biosynthetic enzyme inhibitor

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K05872
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38040:Bioorganic chemistry-related
• Research Institution: Setsunan University
• Principal Investigator: Kato Naoki 摂南大学, 農学部, 准教授 (90442946)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 生合成遺伝子クラスター / 糸状菌二次代謝 / 天然物生合成 / デカリン合成酵素 / 阻害剤

Outline of Final Research Achievements

Natural Product is an important resource in drug discovery. To overcome difficulties in finding new compounds, exploration of the biosynthetic gene clusters (BGCs) of natural products, which are rich in microbial genomes, is necessary. Aim of this study is to establish a new method to find natural products and their BGCs by a combination of genome mining and biosynthetic enzyme inhibitor. We demonstrated that this method was useful for identifying decalin compounds and their BGCs.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

本研究により構築したゲノムマイニングに二次代謝物生合成酵素阻害剤処理を組み合わせた探索系が、特定の部分構造を有する天然物を効率的な探索・収集に有効で、かつ生合成遺伝子クラスターへの紐付けも容易であることを実証した。よって本手法が今後、新規天然物探索の新たな手法の1つとなることが大いに期待できる。