Regulatory mechanisms underlying the generation of intestinal bacteria-reactive antibody in the cecal patches and their modulation by food components

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K05914
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38050:Food sciences-related
• Research Institution: Nihon University
• Principal Investigator: TSUDA Masato 日本大学, 生物資源科学部, 講師 (50525681)
• Co-Investigator(Kenkyū-buntansha): 細野 朗 日本大学, 生物資源科学部, 教授 (70328706)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 腸内細菌 / 腸管関連リンパ組織 / 抗体 / プレバイオティクス

Outline of Final Research Achievements

In this study, we aimed to clarify the mechanisms underlying the generation of intestinal bacteria-reactive IgG antibody in the cecal patches and their regulation by gut microbiota. We showed that cytological features in cecal patches for antibody production that B cells have high ability to generate intestinal bacteria-reactive IgG2b and non-B cells have an ability to help IgG2b class switching from naive B cells. We identified the candidates of bacterial family or genus which contribute to generate IgG2b expressing B cells and the pool of IgG2b antibody in the serum using antibiotics-treated mice and mice purchased from different breeders. In addition, we showed that intervention of prebiotics to mice upregulated the IgG2b responses in the cecal patches.

Free Research Field

食品科学

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は，盲腸リンパ節による抗体産生の仕組みと腸内細菌による調節機序の一端を明らかにした。これらの機構は，感染症や炎症反応などの予防や症状の緩和のために，プレバイオティクスなどの腸内細菌叢を調節する食品成分により大腸における抗体産生応答を制御するための新たな作用点としての応用が期待される。