Dietary induction of intestinal alkaline phosphatase intended to detoxify endotoxin and analysis of its mechanism of action.

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K05936
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38050:Food sciences-related
• Research Institution: Shizuoka University
• Principal Investigator: HINO Shingo 静岡大学, 農学部, 准教授 (70547025)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: アルカリフォスファターゼ / エンドトキセミア / リポポリサッカライド

Outline of Final Research Achievements

It has been suggested that inflammation induced by lipopolysaccharides (LPS) produced by intestinal bacteria and transferred into the bloodstream causes various chronic diseases. Additionally, studies using genetically modified animals have shown that intestinal alkaline phosphatase (IAP) attenuates LPS toxicity. This research aimed to search for dietary components that induce IAP and verify their efficacy. We found that germinated brown rice increases alkaline phosphatase activity and LPS dephosphorylation activity in the intestinal tract. However, the increase in alkaline phosphatase activity was not due to an increase in IAP as previously reported, but was suggested to be due to an increase in another type of alkaline phosphatase.

Free Research Field

栄養化学

Academic Significance and Societal Importance of the Research Achievements

本課題において腸管でのアルカリフォスファターゼ活性(AP)およびLPS分解活性を高める食事成分として新規に発芽玄米を見出した。一方で，このAP活性の上昇は既報から推定されるIAPの増加によるものではないことが示唆された。これらの結果は，既報の経路とは異なるLPS無毒化経路の存在を示唆しており，LPS無毒化による生活習慣病の発症予防および腸管恒常性維持という点において新しい視点を提供すると考えられる。