2022 Fiscal Year Final Research Report
HPLC-ESR investigation to explore potent superoxide radical inhibiter involved in vegetables, fruits and beverages
| Project/Area Number |
20K05937
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38050:Food sciences-related
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| Research Institution | Ehime University (2022)
Kyoto Institute of Technology (2020-2021) |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | スーパーオキシドラジカル / HPLC-ESR / 電子スピン共鳴 / 抗酸化活性 |
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| Outline of Final Research Achievements |
The flow-spin-trapping ESR system combined with the HPLC apparatus (HPLC-ESR) enabled the observation of the ESR chromatograms of column eluates by post-column on-line ST-ESR detection of the DMPO/O2 radical. The current study demonstrates that the intensity and line shape of ESR chromatograms are dominated by SOR scavenging ability and concentration of antioxidants. Furthermore, the total SOR radical scavenging activities of the column-eluted fractions are accurately quantified using the equivalent concentration of caffeic acid ([CAeq.]). HPLC-ESR systems that allow visualization of SOR scavenging functions of column eluates are advantageous for exploring potential SOR scavengers contained in plant-derived water extracts. For example, HPLC-ESR analyses clarified that the most useful SOR scavenging active substances involved in coffee were chlorogenic acid derivatives and these contribution to the total SOR scavenging activity of coffee brew reached about 75%.
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| Free Research Field |
食品物理化学
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| Academic Significance and Societal Importance of the Research Achievements |
最近、優れたスパーオキシドラジカル（以下、SOR）消去活性を備えた青果物、果汁および飲料の積極的な摂取が健康維持に寄与することが広く認知され、SOR消去活性を指標とする青果物および関連食品の開発が続けられている。従来のSOR消去活性評価法では、青果物水溶性成分のSOR消去活性を混合物の状態で計測しているため、主要成分の濃度と活性については不明であった。本研究で確立したHPLC-ESR分析法では、青果物に含まれる主要成分をカラム分離し、それらのSOR消去活性のオンライン分析が可能であり、我が国の食品機能評価の分野への貢献が期待できる。
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