2023 Fiscal Year Final Research Report
Development of efficient purification methods and analyses of health-promoting functions for natural functional ingredients.
| Project/Area Number |
20K05941
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38050:Food sciences-related
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| Research Institution | Fukui Prefectural University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
片野 肇 福井県立大学, 生物資源学部, 教授 (50264685)
神戸 大朋 京都大学, 生命科学研究科, 准教授 (90303875)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | 溶解度分別法 / グリチルリチン / ホウ酸ナトリウム / γ-オリザノール / アセトニトリル / 亜鉛トランスポーター |
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| Outline of Final Research Achievements |
Glycyrrhizin (GL) is a triterpenoid saponin with anti-inflammatory activity. Methanol extract of the licorice powder (GL: 8.5% pure) was solubilized in the borax solution and precipitated by dil. HCl to remove the high polar impurities. Then, the fraction (GL: 31% pure) was treated with Sep-Pak C18 column to remove the low polar impurities (GL: 40% purity), and finally purified by preparative RP-HPLC. However, the total recovery rate was reduced to ca.10% of the initial extract by the column purifications. Then, the ammonia water extract of the licorice (GL: 6.7% purity) was prepared to reduce the low polar impurities. Then, the fraction was solubilized in the borax solution and precipitated by dil. HCl to remove the high polar impurities, resulting in the final fraction (GL: 70% purity, 70% recovery) without columns. In addition, purification method concerning gamma-oryzanol (OZ) was achieved by solubility-based purification, and zinc transporter ZIP4-potentiating activity was found.
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| Free Research Field |
食品科学
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| Academic Significance and Societal Importance of the Research Achievements |
天然素材から生理活性化合物を単離する際には，多段階カラムクロマトが多用されるが，その多くは莫大な時間を要すると共に収率が低い．このため配糖体などの天然化合物の精製標品には、高価なものや市販入手困難なものも多い。そこで本研究では，開発してきたホウ砂や水－有機混合溶媒を用いた簡便な選択的沈殿法等を駆使して，甘草に含まれるグリチルリチン(GL)（トリテルペノイド配糖体）の簡便精製法開発を試み，カラムクロマトグラフィーを使わずに，純度70％の標品を高い回収率（７０％）で取得する方法を開発した．簡便精製法の開発は，希少性の高い天然生理活性化合物を生理活性スクリーニングに供することを可能にする意義を持つ．
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