Elucidation of the structure-function relationship and the sugar-recognition mechanism of novel mutidomain transglucosidases acting on starch

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K05956
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38060:Applied molecular and cellular biology-related
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Tonozuka Takashi 東京農工大学, (連合)農学研究科(研究院), 教授 (50285194)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 糖転移酵素 / マルチドメイン酵素 / GH13 / 澱粉

Outline of Final Research Achievements

This study focuses on analyzing the structure-function relationship of two multi-domain enzymes that act on starch, which aims to develop applications using the enzymes. Both enzymes are glucosyltransferases belonging to the carbohydrate hydrolase family GH13 and consist of N1, N2, N3, A, B, C and D domains. We constructed expression systems for N3-A-B-C and N1, and analyzed the structures and properties. The results indicate that the enzymes have α-1,6-transglucosylation activity, and domains N1 function as starch-binding components.

Free Research Field

応用糖質科学

Academic Significance and Societal Importance of the Research Achievements

澱粉は、植物が生産する主要な栄養貯蔵物質であるとともに、人間にとって重要な食糧であり。澱粉の利用や検出技術の開発は、食品や農学に関する分野の発展に貢献できる。本研究では、二種類のマルチドメイン酵素が、オリゴ糖製造や澱粉の構造の特定に利用できる可能性があることを示した。