Intraspecific cross-compatibility based on the genetic classification of Brassica napus

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K05980
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 39010:Science in plant genetics and breeding-related
• Research Institution: University of Tsukuba
• Principal Investigator: Tsuda Mai 筑波大学, 生命環境系, 助教 (20767511)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 遺伝子組換えナタネ / 遺伝子浸透 / 交雑親和性 / ゲノム

Outline of Final Research Achievements

Transgenic oilseed rape has been identified as a native population in Japan and is the same species as existing oilseed rape in the country, so there is a possibility of genetic penetration of the transgene into the natural environment through hybridisation. However, vegetation surveys around harbours in Japan have not shown gene penetration for more than 10 years. Therefore, genotype analysis and hybridisation affinity studies were conducted to determine the association of nuclear and organelle genotypes and their combinations with hybridisation affinity between domestic native oilseed rape and recombinant oilseed rape. Polymorphism analysis of nuclear and organelle genomes classified them into multiple genotypes. Hybridisation affinity between oilseed rape plants with different genetic backgrounds was low. Future work will clarify the relationship between genotypes, their combinations and hybridisation affinities.

Free Research Field

遺伝育種学

Academic Significance and Societal Importance of the Research Achievements

零れ落ちた遺伝子組換えナタネから日本に自生するナタネやその近縁種への遺伝子浸透の可能性の有無は、遺伝子組換えナタネが国内に輸入されるようになってから長く議論されている。植生調査では遺伝子浸透は確認されないが、科学的にその理由を明確にして示すことは、遺伝子浸透から環境影響を懸念する国民に応えることであり、また今後の遺伝子組換え作物の環境影響を評価するための基礎的知見となりうる。