2023 Fiscal Year Final Research Report
Developing efficient evaluation method for pre-germination flood tolerance in soybean based on new evaluation method for seed imbibition and flood injury
| Project/Area Number |
20K05996
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 39020:Crop production science-related
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | ダイズ / 粒大 / 発芽生長 / 吸水 / 種子内部の隙間 / エバンスブルー / 種皮 / アリューロン層 |
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| Outline of Final Research Achievements |
When comparing the E/A, the resistance evaluation index, setting the seed water content of varieties used for evaluation to the same value is unnecessary. Removing seeds with cracks on the seed coat is also unnecessary if they are not abundant, and E/A can be compared among varieties using stored seeds. These results show that E/A enables efficient evaluation of resistance. Furthermore, since the turbidity of the seed soak solution was shown to be a rapidly measurable resistance evaluation index, the combination of E/A and turbidity will improve the accuracy of resistance evaluation and contribute to the breeding of resistant varieties. It was suggested that the better resistance of small seeds, even of the same variety, is due to the infrequent and narrow gaps inside the seeds. It was also suggested that polyphenols contained in the seed coat of black soybean were involved in improving germination under submerged conditions by inhibiting microbial proliferation during soaking.
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| Free Research Field |
作物学
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| Academic Significance and Societal Importance of the Research Achievements |
種子冠水抵抗性の評価指標としてE/Aは測定容易で、種子含水率の厳密な調整不要、裂皮粒もその頻度によっては除去不要であるなどを明らかにした。抵抗性評価には種子含水率調整が必須とされていたため、E/Aは実用性に優れる。また、迅速測定可能な種子浸漬液の濁度も抵抗性指標として利用可能であった。E/Aと濁度の組合せで抵抗性評価の精度向上と抵抗性選抜の効率化が期待でき、実用的な成果を得た。同一品種でも小粒の方が抵抗性が高いのは、種子内部に隙間がある頻度が低く、隙間があっても狭いためであることを明らかにした。播種用種子として大粒を除外すれば抵抗性の向上策になり得る。これは、早急な対策としての意義が大きい。
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