Identification and functional analysis of antibacterial proteins isolated for plant pathogen control.

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K06059
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 39040:Plant protection science-related
• Research Institution: Sojo University
• Principal Investigator: Ekino Keisuke 崇城大学, 生物生命学部, 教授 (30310030)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: Bacillus thuringiensis / 植物病原菌 / そうか病 / 抗菌タンパク質

Outline of Final Research Achievements

Among Bacillus thuringiensis isolated for the purpose of controlling plant pathogenic fungi, strain A297 exhibits antibacterial activity against the potato scab pathogenic Streptomyces strains. As a result of purifying the antibacterial protein produced by this strain, it became clear that it produced two kinds of antibacterial proteins. These antibacterial proteins have no homology with proteins whose functions have been elucidated so far, and were found to be novel functional proteins. The mechanism of action of the antibacterial protein was analyzed by fluorescence staining using the presence or absence of membrane damage as an index. As a result of microscopic observation, it was suggested that the antibacterial protein isolated in this research project damaged the cell membrane of the potato scab pathogenic Streptomyces strains and inhibited its growth.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

植物病原菌の微生物防除を目的に、ジャガイモそうか病の病原菌に対して抗菌活性を示す微生物を分離した。この微生物が生産する2種類の抗菌タンパク質が、ジャガイモそうか病菌の生育を阻害することが明らかとなった。また、今回発見した抗菌タンパク質はこれまで知られていない新規なタンパク質であり、その作用機構はジャガイモそうか病菌の細胞膜の損傷であることが示唆された。微生物による植物病の防除は、化学農薬の低減に寄与し、環境への負荷を減らすことができる。