Regulation of skeletal muscle energy metabolism and differentiation.

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K06359
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 42010:Animal production science-related
• Research Institution: Tokai University (2021-2022); Utsunomiya University (2020)
• Principal Investigator: Sato Yusuke 東海大学, 農学部, 特任准教授 (50589520)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 骨格筋 / エネルギー代謝 / 分化 / ミトコンドリア / Apobec2

Outline of Final Research Achievements

Skeletal muscles are composed of multinucleated myofibers that differentiate and fuse from mononuclear myoblasts and are the largest energy metabolizing organs in the body. In this study, the mechanism by which muscle cell differentiation and energy metabolism are controlled by the deaminase enzyme Apobec2 was investigated. Forced expression of Apobec2 in C2C12 myoblasts significantly suppressed the differentiation of myoblasts into myotubes. In addition, knockdown of Apobec2 by siRNA increased mitochondrial respiration in C2C12 myoblasts. These results suggest that Apobec2 may regulate both differentiation and energy metabolism in skeletal muscle cells.

Free Research Field

代謝学、畜産物利用学

Academic Significance and Societal Importance of the Research Achievements

筋細胞の分化・代謝の制御機構を理解することができれば、骨格筋量の維持・増加あるいは性質をコントロールできるようになる。本研究により、筋細胞の代謝・分化の制御機構の一旦が明らかになった。研究成果は、サルコペニアの予防やスポーツ科学に貢献するだけでなく、昨今注目が集まる培養肉の開発に資すると考える。