2022 Fiscal Year Final Research Report
Development of atopic dermatitis models using humanized mice for eosinophil target therapy
Project/Area Number |
20K06477
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 42040:Laboratory animal science-related
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Research Institution | Central Institute for Experimental Animals |
Principal Investigator |
Ito Ryoji 公益財団法人実験動物中央研究所, 実験動物応用研究部, 室長 (60425436)
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | アレルギー / ヒト化マウス |
Outline of Final Research Achievements |
The aim of this study is to develop a human cell-mediated model of atopic dermatitis using humanized mice and to analyze the function of human cells while establishing a preclinical model. Initially, we examined whether subcutaneous administration of hIL-33 induced atopic dermatitis in hIL-3/GM-CSF/IL-5 triple transgenic mice; however, unfortunately, these mice did not demonstrate T cell infiltration or epidermal thickening. Subsequently, we attempted to induce dermatitis by applying oxazolone to hIL-4 transgenic mice, which are capable of differentiating into Th2 cells. Consequently, we observed a significant infiltration of T cells in the skin of these transgenic mice. These findings suggest that the hIL-4 transgenic mouse is a useful model for human atopic dermatitis.
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Free Research Field |
アレルギー、実験動物、ヒト化マウス
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Academic Significance and Societal Importance of the Research Achievements |
本研究の学術的、社会的意義は、ヒト化マウスを用いてアトピー性皮膚炎を始めとしたアレルギーモデルを開発し、ヒト細胞の機能解析および前臨床モデルを確立し、医学、創薬研究に貢献することにある。ヒトアトピー性皮膚炎の病態解明や新たな治療法の開発において、皮膚炎病態を再現したヒト化動物モデルは極めて有益であり、アトピー性皮膚炎患者の生活の質の向上や、効果的な治療法の開発による医療費の削減につながる可能性がある。また本研究のような免疫系ヒト化マウスの開発、改良を重ねることで、他の免疫関連疾患の研究にも応用できる可能性があり、さらなる疾患治療の進歩に寄与することが期待できる。
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