The importance of alternative polyadenylation during cortical development

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K06894
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 46020:Anatomy and histopathology of nervous system-related
• Research Institution: Gifu Pharmaceutical University
• Principal Investigator: Soumiya Hitomi 岐阜薬科大学, 薬学部, 講師 (20548713)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 大脳皮質 / 選択的ポリA付加反応

Outline of Final Research Achievements

To visualize the alternative polyadenylation, we constructed vectors for polyadenylation site with destabilized Venus. However, the longer the back sequence, the weaker the fluorescence intensity became and could not be evaluated. The mutants of X, the molecule responsible for the alternative polyadenylation, altered the migration pattern of cortical progenitors. In addition, a system to evaluate the changes in complex formation of X was created, and it was revealed that the complex formation of X changes with neural differentiation.

Free Research Field

神経科学

Academic Significance and Societal Importance of the Research Achievements

選択的ポリA付加反応は遺伝子形状を変化させ、遺伝子発現の多様性を作り出す。分化した細胞ほど長い３‘UTRを持つことが知られているが、その制御機構は明らかになっていない。本研究では、高次脳機能を司る大脳皮質構築過程に着目し、選択的ポリA付加反応の変動の挙動の一端を評価するツールを作製した。本ツールにより遺伝子発現の多様性が高次脳機能の出現を支える機構の一端を明らかにできると考えられる。