Development of enhanced throughput LC/MS assays of steroid hormones based on sample and analyte-multiplexing

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K06990
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related
• Research Institution: Tokyo University of Science
• Principal Investigator: Higashi Tatsuya 東京理科大学, 薬学部薬学科, 教授 (90272963)
• Co-Investigator(Kenkyū-buntansha): 石毛 崇之 千葉大学, 医学部附属病院, 臨床検査技師 (30757315)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 検体・項目多重化 / ハイスループット / ステロイドホルモン / LC/MS / 臨床検査 / 誘導体化 / MSタグ

Outline of Final Research Achievements

The novel methods employing multiple MS tags, named as “SAM (Sample and Analyte-Multiplexing)”, were proposed and developed to enhance the throughput of the LC/MS-based tests for some steroid hormones, which are currently used as the diagnostic and therapeutic markers for primary aldosteronism, late-onset hypogonadism syndrome and osteoporosis. By the SAM methods, the total LC/MS run times were reduced by up to 60%, compared with those in the previous methods. Furthermore, the newly developed methods enabled the simultaneous measurements of two steroid hormones which are hitherto measured by the two separate methods; this capability further enhanced the assay throughput.

Free Research Field

分析科学

Academic Significance and Societal Importance of the Research Achievements

本研究により，ステロイドホルモンのLC/MS検査の実用化を阻んでいる「スループットの低さ」が，適切にデザインされたMSタグを利用する「検体・項目多重化 (SAM) 技術」によって克服できることが示された．すなわち，本成果は各病院・検査機関が現有するLC/MS装置でも検査のハイスループット化が可能であることを証明しており，ステロイドホルモン検査には正診度及び医療経済性に優れるLC/MSを第一手法とすべきことを強く提言するものである．