Functional analysis of type II membrane protein CKAP4 at the individual level

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K07357
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 49010:Pathological biochemistry-related
• Research Institution: Osaka University
• Principal Investigator: Takeshi Harada 大阪大学, 大学院医学系研究科, 助教 (30362768)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: CKAP4 / ミトコンドリア / 小胞体 / 脂質

Outline of Final Research Achievements

CKAP4 was identified more than 25 years ago as a type II single transmembrane protein with a molecular weight of 63 KDa that is mainly localized in the endoplasmic reticulum and is involved in the structural maintenance of the ER. However, there have been no reports on CKAP4 KO mice other than ours, and functional analysis at the individual level has not been performed at all. By using CKAP4 KO mice, we were able to clarify how the various functions of CKAP4, which have been clarified in cultured cell-based experiments, are related to the regulation of glucose and lipid metabolism in the individual.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

CKAP4の多彩な機能が個体における糖・脂質代謝調節に如何に関連するかを明らかにすることにより、これまで明らかになっていない小胞体構造タンパク質と糖・脂質代謝調節との関連が明らかとなった。この研究から、糖尿病や脂質異常症・高血圧症・心血管疾患などの生活習慣病の病態解明につながることが期待され、医療という側面からも社会的意義も大きく、創造性の高い研究であったと考えている。