Molecular pathology evaluation method of PD-L1/PD-L2 focusing on structural and splicing abnormalities

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K07399
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 49020:Human pathology-related
• Research Institution: Japanese Foundation for Cancer Research
• Principal Investigator: SAKATA Seiji 公益財団法人がん研究会, がん研究所 分子標的病理プロジェクト, 研究員 (00617433)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: バイオマーカー

Outline of Final Research Achievements

Targeted capture sequencing data of lymphoma specimens with high PD-L1 expression, their PD-L1 expression patterns by immunohistochemistry, and FISH findings were examined in detail. Samples in which PD-L1 genomic structural abnormalities were detected by targeted capture sequencing had a high rate of abnormal signal patterns by FISH. Almost all of the specimens that showed an abnormal expression pattern of PD-L1 by immunohistochemistry showed PD-L1 genomic structural abnormalities. Combined search of immunohistochemistry and FISH results showed a high detection rate of PD-L1 genomic structural aberrations.

Free Research Field

人体病理学

Academic Significance and Societal Importance of the Research Achievements

ゲノム構造異常に伴うPD-L1遺伝子の3´非翻訳領域の変異の検索には、一般的にターゲットキャプチャーシーケンスでの検索が必要となるが、経験やスキルが必要で費用が高く、利用可能な機関が限られる。免疫染色およびFISHはより簡便に行うことができ、検体量の少ない場合でも検索可能なため、ゲノム構造異常に伴うPD-L1遺伝子の3´非翻訳領域の変異の検索に免疫染色とFISHが有用であったことは社会的に意義がある。