2022 Fiscal Year Final Research Report
Molecular pathology evaluation method of PD-L1/PD-L2 focusing on structural and splicing abnormalities
Project/Area Number |
20K07399
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 49020:Human pathology-related
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Research Institution | Japanese Foundation for Cancer Research |
Principal Investigator |
SAKATA Seiji 公益財団法人がん研究会, がん研究所 分子標的病理プロジェクト, 研究員 (00617433)
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | バイオマーカー |
Outline of Final Research Achievements |
Targeted capture sequencing data of lymphoma specimens with high PD-L1 expression, their PD-L1 expression patterns by immunohistochemistry, and FISH findings were examined in detail. Samples in which PD-L1 genomic structural abnormalities were detected by targeted capture sequencing had a high rate of abnormal signal patterns by FISH. Almost all of the specimens that showed an abnormal expression pattern of PD-L1 by immunohistochemistry showed PD-L1 genomic structural abnormalities. Combined search of immunohistochemistry and FISH results showed a high detection rate of PD-L1 genomic structural aberrations.
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Free Research Field |
人体病理学
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Academic Significance and Societal Importance of the Research Achievements |
ゲノム構造異常に伴うPD-L1遺伝子の3´非翻訳領域の変異の検索には、一般的にターゲットキャプチャーシーケンスでの検索が必要となるが、経験やスキルが必要で費用が高く、利用可能な機関が限られる。免疫染色およびFISHはより簡便に行うことができ、検体量の少ない場合でも検索可能なため、ゲノム構造異常に伴うPD-L1遺伝子の3´非翻訳領域の変異の検索に免疫染色とFISHが有用であったことは社会的に意義がある。
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