Genetic analysis of initial and recurrent DLBCLs

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K07416
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 49020:Human pathology-related
• Research Institution: Tokai University
• Principal Investigator: Nakamura Naoya 東海大学, 医学部, 教授 (50227922)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: DLBCL / IGH / Gene expression

Outline of Final Research Achievements

A total of 23 cases (46 samples including initial and recurrent samples) of Diffuse large B-cell lymphoma (DLBCL) were analyzed by molecular techniques. PCR and Sanger sequencing of immunoglobulin heavy chain gene variable region and CDR regions showed 9 cases with same B-cell clone, 11 cases with different clones and 3 cases with undetermined origin. Gene expression profiling (GEP) was analyzed in all samples and showed high expression genes in initial samples and recurrent samples in all cases, respectively. GEP in 9 cases with same clone showed high expression genes in initial samples and recurrent samples, respectively. Those genes were specific for true recurrent samples of DLBCL. Therefore, we demonstrated important genes in recurrent DLBCL.

Free Research Field

人体病理学

Academic Significance and Societal Importance of the Research Achievements

これまで明らかになっていなかった、再発びまん性大細胞型B細胞リンパ腫（DLBCL）の疑問、初発B細胞クローンが再発するのか、初発とは異なる新たなB細胞クローンが腫瘍化するのか、また、同じクローンで再発する場合、どのようなメカニズムがあるか、に新たな知見を得、DLBCLの腫瘍発生メカニズムの解明がさらに進むものと期待できる。この結果は、再発DLBCLの治療、薬剤の選択、治療方針の決定に大きな影響を及ぼす可能性がある。さらに、DLBCL全体の予後改善をもたらす可能性があり、がん患者さんの予後の改善、QOLの向上を望むことができる。