2023 Fiscal Year Final Research Report
Construction of evaluation system for novel PPI inhibitors based on the Hippo pathway in the model organism C.elegans
| Project/Area Number |
20K07425
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 49030:Experimental pathology-related
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| Research Institution | Oita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
長谷川 英男 大分大学, 医学部, 名誉教授 (00126442)
松浦 恵子 大分大学, 医学部, 教授 (00291542)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | Hippo pathway / モデル生物 / 線虫 / 阻害剤 |
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| Outline of Final Research Achievements |
The Hippo pathway is a signaling pathway that is evolutionarily conserved in many animals and is important for biological activities. In this study, we conducted experiments using Caenorhabditis elegans, a species of nematode, as a model organism with the aim of constructing a biological model system that enables the discovery and evaluation of drugs that regulate the Hippo pathway. We found that when C. elegans was cultured in liquid medium and genes were suppressed by RNA interference, the morphology of the adult worms was mutated. The addition of inhibitors also caused changes in morphology and lifespan, and the effects varied depending on the type of gene suppressed. These results indicate that it is possible to conduct experiments to control gene expression in C. elegans and verify the effects of drugs.
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| Free Research Field |
生物学
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| Academic Significance and Societal Importance of the Research Achievements |
線虫はマウスなどに比べると飼育に大きな設備を必要とせず、短期間に大量に培養することができる。本研究では線虫を用いることで、より安価で網羅的な薬剤評価を行う生体モデルシステムの実現可能性を示すことができたものと考える。Hippo pathwayは細胞の増殖、腫瘍の抑制、器官の大きさ、再生といった重要な機能に関わるシグナル伝達経路であり、モデル線虫の表現型に変化をもたらす薬剤の探索は医薬品創出やガン研究に寄与することが期待できる。
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