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2022 Fiscal Year Final Research Report

Study for novel biomarker in liquid biopsy on on carcinogenic pathways induced by driver mutations

Research Project

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Project/Area Number 20K07671
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 50020:Tumor diagnostics and therapeutics-related
Research InstitutionCenter for Clinical and Biomedical Research, Sapporo Higashi Tokushukai Hospital

Principal Investigator

Ono Yusuke  医療法人徳洲会札幌東徳洲会病院医学研究所, ゲノム診断研究部, 部門長 (40742648)

Co-Investigator(Kenkyū-buntansha) 水上 裕輔  旭川医科大学, 医学部, 教授 (30400089)
唐崎 秀則  医療法人徳洲会札幌東徳洲会病院医学研究所, がん生物研究部, 客員研究員 (50374806)
Project Period (FY) 2020-04-01 – 2023-03-31
Keywordsリキッドバイオプシー / ドライバー変異 / 遊離RNA / デジタルPCR / 膵癌 / IPMN
Outline of Final Research Achievements

In this study, we searched for new biomarkers targeting RNAs linked to driver genes using pancreatic cancer as a model. We generated KRAS mutant, GNAS mutant, KRAS/GNAS mutant, and wild-type strains of these genes in two different pancreatic ductal epithelial cell lines (HPDE, HPNE) and analyzed their expression patterns by RNA-seq. Furthermore, an effective method for collecting and purifying unstable plasma cell-free mRNA was validated, and a highly sensitive expression detection system using digital PCR was constructed. cfRNA was extracted from 35 PDA patient samples, and the amounts present were compared after quantification.

Free Research Field

腫瘍診断学

Academic Significance and Societal Importance of the Research Achievements

国内現行の血漿CGP検査は、現時点では進行癌患者を対象とし、検査目的は抗がん剤・分子標的薬の選択に限定される。研究ベースでは遺伝子変異にメチル化検出を組み合わせた包括的パネルが発表され、またmiRNAについて多癌腫のmiRNA検出パネルも検討されてきた。一方で本研究の対象としたmRNA、ncRNAはアポトーシス/壊死腫瘍細胞からの情報とシグナル伝達などの活性腫瘍細胞からの情報の両方、すなわち癌に関する直接のリアルタイム情報を取得できる可能性があり、本研究で目指した効果的な遊離RNAの回収と検出系を用いることにより、上記パネルと比較すると、特定の癌腫を早期で捉えるバイオマーカーになり得る。

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Published: 2024-01-30  

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