Elucidation of the molecular biological mechanisms of irradiated cells cultured under normoxic or hypoxic conditons

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K08065
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 52040:Radiological sciences-related
• Research Institution: National Institutes for Quantum Science and Technology
• Principal Investigator: Liu Cuihua 国立研究開発法人量子科学技術研究開発機構, 量子医科学研究所 重粒子線治療研究部, 主任研究員 (00512427)
• Co-Investigator(Kenkyū-buntansha): 平山 亮一 国立研究開発法人量子科学技術研究開発機構, 重粒子線治療研究部, 研究統括 (90435701)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 放射線 / 重粒子線 / 低酸素培養 / miRNA / トランスフェクション

Outline of Final Research Achievements

Many miRNAs in mouse kidney　primary cultured cells cultured in a hypoxic condition were found to be changed by more than 2 times or less than 0.5 times compared with the cells cultured in a normoxic conditions after irradiation. Specifically, in the case of mmu-miR-210-3p and mmu-miR-210-5p, regardless of the quality of radiation, the fold changes was 2 to 7 times higher than those cells cultured in a normoxic conditions. After transfected the mimic or inhibitor of these two kinds of miRNA into mouse kidney primary cultured cells, the cell proliferation and radiation sensitivity were altered. The results showed that the miR-210-3p and miR-210-5p maybe associated with cell radiosensitivity or cell proliferation.
On the other hand, the numbers of fold-changed miRNAs in human glioblastoma cells (U87MG) was lower than that mouse kidney primary cells cultured under the same conditions.

Free Research Field

放射線生物影響

Academic Significance and Societal Importance of the Research Achievements

本研究では生体内低酸素環境を模擬した実験系において、がん細胞ならびに正常組織細胞のmiRNA発現プロファイルを解析する。miRNA発現スクリーニングにより低酸素環境で培養した細胞の放射線抵抗性やNHEJ修復忠実度に関するメカニズムを明らかにする。これらの実験により、生体内を模擬した低酸素環境下でどのような因子が放射線応答に関わっているかを調べ、放射線治療生物学の重要な基礎データを蓄積する。そして新たな有・低酸素生体応答の研究基盤を構築する。