Examination of novel prognostic markers related to spontaneous regression and differentiation of neuroblastoma

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K08212
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 52050:Embryonic medicine and pediatrics-related
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Iehara Tomoko 京都府立医科大学, 医学(系)研究科(研究院), 教授 (20285266)
• Co-Investigator(Kenkyū-buntansha): 柳生 茂希 京都府立医科大学, 医学(系)研究科(研究院), 助教 (10572547) ; 吉田 秀樹 京都府立医科大学, 医学(系)研究科(研究院), 助教 (10643546) ; 菊地 顕 京都府立医科大学, 医学(系)研究科(研究院), 特任講師 (40453104) ; 宮地 充 京都府立医科大学, 医学(系)研究科(研究院), 助教 (40584983) ; 土屋 邦彦 京都府立医科大学, 医学(系)研究科(研究院), 講師 (90381938)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 神経芽腫 / 分化

Outline of Final Research Achievements

Using neuroblastoma patient sera, we found four miRNA candidates that were predominantly expressed at low risk. In a neuroblastoma cell line differentiation model using retinoic acid, we confirmed the presence of this candidate miRNA in the culture supernatant and exosomes. When candidate miRNA mimic and miRNA inhibitor were introduced into neuroblastoma cell lines, morphological changes such as differentiation and proliferation of neuroblastoma cells were not observed. As an indicator of differentiation, neurofilament was measured and transfection of one of the candidates increased neurofilament and repression decreased it, suggesting that this X-miRNA may be related in induction of differentiation.

Free Research Field

小児がん

Academic Significance and Societal Importance of the Research Achievements

神経芽腫NBの特徴は、予後の多様性にあり、自然退縮するNBのマーカーは知られておらず、これを解明することで、無治療可能な腫瘍の判別が可能となり、新たな治療法の開発に繋げられ、重要な問いとなると考えられる。新たな非侵襲的な診断マーカー、予後マーカーに使えるだけでなく、NBの病態解明、新たな分子標的療法の開発につながることが期待出来る。