Verification of universal oxidative stress regulation ability by transcriptional coactivator PDIP1 and search for target molecules

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K08302
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53010:Gastroenterology-related
• Research Institution: Gunma University
• Principal Investigator: Ken Sato 群馬大学, 医学部, 非常勤講師 (40396619)
• Co-Investigator(Kenkyū-buntansha): 山崎 勇一 群馬大学, 医学部附属病院, 講師 (00582404) ; 堀口 昇男 群馬大学, 大学院医学系研究科, 講師 (10550022)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: PDIP1 / 転写共役活性化因子 / アルコール性肝炎 / ノックアウトマウス / 酸化ストレス

Outline of Final Research Achievements

We compared PDIP1 knockout (KO) mice with wild type (WT) mice in an alcoholic hepatitis model. Blood biochemical tests showed that serum ALP and TG levels were significantly lower in KO mice than in WT mice, but there were no differences in serum ALT, AST, total bilirubin levels, and so on between them. Therefore, we compared the effects of alcohol-loaded and alcohol-unloaded WT mice to check the quality of our experiment. Then, we found that serum ALP and cholesterol levels were significantly lower in alcohol-loaded mice, while serum AST, ALT, bilirubin, TG levels, and so on were not significant different between them, which suggests that the experimental system may have been insufficiently established. Histopathological examination revealed no obvious difference between WT and KO mice in the alcoholic hepatitis model.

Free Research Field

消化器内科学

Academic Significance and Societal Importance of the Research Achievements

研究成果に関しては、残念ながらpreliminaryな結果となっており、今回の結果は、実験系の確立には至っていない可能性を示唆しています。研究テーマの真の結果を求めるには、再実験で、実験の条件などの変更等を行っていく必要があり、実験の難しさを痛感しました。学術的意義や社会的意義に関しては再実験の結果論文化等で今後明らかにできれば幸いと思っております。