2023 Fiscal Year Final Research Report
Clinical applications of plasma cell-free DNA as a predictor of prognosis after acute heart failure and myocardial infarction
| Project/Area Number |
20K08436
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 53020:Cardiology-related
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
藤林 幸輔 金沢医科大学, 医学部, 講師 (10633323)
藤原 純子 島根大学, 学術研究院医学・看護学系, 助教 (20346381)
若狭 稔 金沢医科大学, 医学部, 講師 (50468980)
竹下 治男 島根大学, 学術研究院医学・看護学系, 教授 (90292599)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | cell-free DNA / acute heart failure / apoptosis |
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| Outline of Final Research Achievements |
The source of cell-free DNA (cfDNA) in patients with acute heart failure (AHF) is unknown. Therefore, we evaluated the transmayocardial arteriovenous differences of plasma cfDNA concentrations in patients with AHF.We enrolled 30 patients with AHF. Blood samples were obtained simultaneously at aortic root (AO) and at coronary sinus (CS). We evaluated the plasma levels of transmyocardial gradient of cfDNA concentration (delta cfDNA). Myocardial samples were subjected to histological examination.The mean levels of delta cfDNA in 18 patients were 3.1±3.5 microg/ml (positive group), in the other 12 patients were -3.1±2.4 microg/ml (negative group). In the histological evaluation, 7 of 18 patients (39%) in the positive group showed cardiac fibrosis, 9 of 12 patients (75%) in the negative group showed cardiac fibrosis.
We postulated myocardial apoptosis is occurring in the positive group without cardiac fibrosis and gradually would cause cardiac fibrosis.
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| Free Research Field |
循環器内科
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| Academic Significance and Societal Importance of the Research Achievements |
急性心不全患者において冠静脈洞と大動脈基部のcfDNA濃度差の検討から、cfDNAが心臓で産生されたものかどうか、またその臨床的な意義は明らかにはならなかった。さらにcfDNAが負の値になる合理的な理由も説明ができなかった。したがってcfDNAが心疾患でどのような意義を持っているのかを今後さらに明らかにし、そのための研究方法を見出す必要がある。
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