Comprehensive Fundamental Research on the Onset and Development of Pneumoconiosis Using TAS-Seq

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K08556
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53030:Respiratory medicine-related
• Research Institution: Japan Organization of Occupational Health and Safety Japan Bioassay Research Center
• Principal Investigator: Yamano Shotaro 独立行政法人労働者健康安全機構 日本バイオアッセイ研究センター(試験管理部、病理検査部), その他部局等, 室長補佐 (80614528)
• Co-Investigator(Kenkyū-buntansha): 杉原 英志 藤田医科大学, オープンファシリティセンター, 准教授 (50464996) ; 七野 成之 東京理科大学, 研究推進機構生命医科学研究所, 助教 (70822435)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: じん肺 / シングルセル解析 / scRNAseq / インジウム / SITO / シリカ / RCS / 職業性疾患

Outline of Final Research Achievements

The identification of pneumoconiosis risk substances and the elucidation of their pathogenic mechanisms and the development of rapid and efficient short-term evaluation methods in an organic manner are urgent issues and important research subjects to be promoted. Therefore, in this study, we generated rat pneumoconiosis by injecting Silica or SITO into rats and performed TAS-Seq analysis to analyze the pathogenesis of pneumoconiosis at a single-cell resolution. As a result, we succeeded in identifying characteristic new cell subsets that constitute pneumoconiosis onset and progression. The clarification of the functions and regulatory mechanisms of these cell groups is expected to accelerate fundamental research on pneumoconiosis that will contribute to occupational safety and health in the future.

Free Research Field

呼吸器

Academic Significance and Societal Importance of the Research Achievements

研究開発が遅れている職業性呼吸器疾患の病態解明に対して、疾患の発症・進展に重要な役割を担う可能性のある新規細胞種を同定することができ、学術的意義の高い成果が得られた。加えて、これらの特徴的な細胞に対して介入方法を将来検討できるようになり、職業性肺疾患罹患者の予防、治療等に資する基盤的成果が提供できた。