The 3D imaging and experimental model construction of Palmoplantar pustulsosii

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K08691
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53050:Dermatology-related
• Research Institution: Ehime University
• Principal Investigator: Masamoto Murakami 愛媛大学, 医学系研究科, 特任教授 (20278302)
• Co-Investigator(Kenkyū-buntansha): 八束 和樹 愛媛大学, 医学部附属病院, 助教(病院教員) (30844766) ; 川上 良介 愛媛大学, 医学系研究科, 准教授 (40508818) ; 森 秀樹 愛媛大学, 医学系研究科, 講師 (60325389)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 掌蹠膿疱症 / 三次元イメージング / ソルバトクロミック色素 / バイオイメージング

Outline of Final Research Achievements

Bioimaging in the blister environment of palmoplantar pustulosis using two-photon excitation microscopy was successfully performed to observe the lesion environment in three dimensions. By establishing a two-photon microscopic observation method using a new solvatochromic dye in combination with a transparency agent, we were able to obtain a detailed three-dimensional histological image that is morphologically comparable to existing HE-stained histopathological images (reported in the previous year). The new technique dramatically shortened the time required to prepare and diagnose pathology using conventional HE staining (from 1-2 weeks to 3 days), and the staining method is extremely simple and does not require any special staining equipment.

Free Research Field

皮膚科学

Academic Significance and Societal Importance of the Research Achievements

透明化剤と新規ソルバトクロミック色素による新規蛍光組織染色方法を考案し、二光子顕微鏡による観察方法を組み合わせることで、これまで見ることのできなかった組織三次元構築を既存病理組織染色方法と同レベルで描出することに世界で初めて成功した。さらにin vitroのみならずin vivoでもこの観察方法が応用できる可能性が明らかとなり、さらに技術の発展性が期待される。