Exploration of the role of a novel G-coupled protein receptor in normal hematopoiesis and hematological malignancy

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K08706
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 54010:Hematology and medical oncology-related
• Research Institution: The University of Tokyo
• Principal Investigator: Fukuyama Tomofusa 東京大学, 医科学研究所, 助教 (10300906)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 造血器腫瘍 / Gタンパク質共役受容体 / RUNX1 / RUNX1T1 / 選択的スプライシング / AML1 / MTG8

Outline of Final Research Achievements

1. We explored the role of a novel GPCR in hematopoietic tumors. We expressed this novel GPCR in mouse myeloid tumor cells and analyzed its role. We also examined the role of this novel GPCR in the survival of GPCR-expressing cells. Furthermore, RNA-seq analysis provided data to infer a new signal transduction pathway by this GPCR.
2. We investigated the role of this GPCR in normal hematopoietic cells. Peripheral blood samples from GPCR knockout mice were analyzed.

Free Research Field

血液内科学

Academic Significance and Societal Importance of the Research Achievements

今回新たに見いだした造血器腫瘍特異的に発現しているGPCRは、細胞の様々な機能に関与して腫瘍化に寄与していると考えられた。一方正常造血における本GPCRの役割も検討し、本GPCRを阻害することは新たな造血器腫瘍の標的戦略と考えられた。