Elucidation of the molecular mechanisms responsible for IPMNs progression

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K09025
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55020:Digestive surgery-related
• Research Institution: Asahikawa Medical College
• Principal Investigator: IMAI KOJI 旭川医科大学, 医学部, 講師 (10516420)
• Co-Investigator(Kenkyū-buntansha): 水上 裕輔 旭川医科大学, 医学部, 教授 (30400089) ; 唐崎 秀則 医療法人徳洲会札幌東徳洲会病院医学研究所, 外科的消化器病疾患研究部, 客員研究員 (50374806)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: IPMN / 膵癌 / 病理マッピング / ゲノムプロファイリング / Visium空間トランスクリプトーム解析

Outline of Final Research Achievements

We conducted a precise pathological mapping and multiregional sequencing analysis on a surgically resected pancreas to identify characteristics of IPMN that may lead to invasive cancer. We marked the specimens into different components with varying histological grades and epithelial subtypes. Each region, including microscopic lesions in the normal-looking pancreas, underwent targeted sequencing, enabling us to estimate clonal relatedness and spatial distribution in each area using minimal mutation markers. Our goal is to investigate the mechanisms behind malignant transformation by increasing the histological grade of neoplastic lesions and performing spatial expression analysis to identify any factors that cannot be explained by a few driver mutations.

Free Research Field

消化器（外科）

Academic Significance and Societal Importance of the Research Achievements

膵管内乳頭粘液性腫瘍を含む膵嚢胞性疾患は膵癌の危険因子とされるが、その多くが臨床的には良性と考えられる。本腫瘍の悪性化機構の理解を通じて悪性化しやすい病変の特性を定義することは、膵癌の早期診断はもとより、外科切除の適応と時期を適切に判断する根拠を提示し、このことは国民の健康福祉の観点から大きな意義がある。