Development of therapy for improving liver regeneration disorders associated with obstructive jaundice through intestinal microbiota metagenome analysis

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K09101
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55020:Digestive surgery-related
• Research Institution: Shinshu University
• Principal Investigator: Shimizu Akira 信州大学, 学術研究院医学系(医学部附属病院), 准教授 (00447773)
• Co-Investigator(Kenkyū-buntansha): 副島 雄二 信州大学, 学術研究院医学系, 教授 (30325526) ; 安川 紘矢 信州大学, 医学部, 特任助教 (30868071) ; 野竹 剛 信州大学, 学術研究院医学系(医学部附属病院), 助教 (40645511)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 腸内細菌叢 / 胆汁鬱滞モデル / 肝切除

Outline of Final Research Achievements

Based on the hypothesis that obstructive jaundice with biliary stasis, which causes dramatic changes in the enterohepatic circulation, disrupts the gut microbiota and impairs liver regeneration capacity, we conducted the following study.
We created a biliary stasis model using common bile duct ligation and compared the gut microbiota diversity with a control model. As a result, while a difference in alpha diversity was observed on Day 4 in the biliary stasis model, no significant changes in beta diversity were confirmed at each time point between the control and biliary stasis models.
Additionally, models with 80% hepatectomy added to both the control and biliary stasis models were created. When comparing the ratio of remnant liver weight to total liver weight on postoperative day 2, no significant difference was observed as initially expected.

Free Research Field

消化器外科

Academic Significance and Societal Importance of the Research Achievements

胆汁鬱滞という腸肝循環に大きな変化と影響を及ぼす病態における腸内細菌叢の変化について、その一端を明らかにすることができたが、コントロールと比較して著明な細菌叢変化を生じている証拠を明らかにすることはできず、また肝再生抑制効果に関しても検証は不十分であり、今後の課題と考えられた。