Evaluation of the Effectiveness of Minimally Invasive Cell Therapy in Post-Cardiac Arrest Consciousness Disorders: Aiming for Patient Social Reintegration

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K09274
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55060:Emergency medicine-related
• Research Institution: Nippon Medical School
• Principal Investigator: Yokobori Shoji 日本医科大学, 大学院医学研究科, 大学院教授 (70449271)
• Co-Investigator(Kenkyū-buntansha): 須田 智 埼玉医科大学, 医学部, 教授 (00366733) ; 佐々木 和馬 獨協医科大学, 医学部, 助教 (30832266) ; 山田 真吏奈 日本体育大学, 保健医療学部, 准教授 (70508621) ; 阪本 太吾 日本医科大学, 医学部, 助教 (90587073)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 心停止後症候群

Outline of Final Research Achievements

In this study, we were able to establish and stabilize a rat model of post-cardiac arrest. Specifically, we conducted cannulation of the rat's tail artery under anesthesia, followed by endotracheal intubation. We then administered muscle relaxants to suppress spontaneous breathing, clamped the tube to induce asphyxia, and resumed ventilation after 5 minutes. Subsequently, we successfully created a post-cardiac arrest model by performing chest compressions to restore cardiac activity.

Furthermore, we are in the stage of considering therapeutic approaches such as neural stem cells and neurotrophic factors. For example, we are currently transplanting neural stem cells (San-bio SB-623) stereotactically into the brain ventricle and observing the recovery of neural function. Additionally, we aim to further establish precise behavioral assessment experiments and refine techniques for cell transplantation.

Free Research Field

救急医学

Academic Significance and Societal Importance of the Research Achievements

本研究はラット蘇生後脳症モデルを作成し、ヒト骨髄由来間葉系幹細胞（SB623）を経鼻的に投与する。in vivoによる電気生理学的評価、行動実験および組織学的評価を行うことで、細胞医薬による神経組織再生・保護が蘇生後脳症に対する機能転帰の改善に貢献するかを検討する。本研究は蘇生後脳症の治療において低侵襲的な細胞治療を応用する初めての試みとなった。