2022 Fiscal Year Final Research Report
Analysis of Glymphatic system in ideopathic normal pressure hydrocephalus rat
Project/Area Number |
20K09344
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 56010:Neurosurgery-related
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Research Institution | University of Fukui |
Principal Investigator |
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | 正常圧水頭症 / ラット / モデル |
Outline of Final Research Achievements |
Under general anesthesia with in halation of 0.2% isoflurane, the head of 230-250g-weighted male SD rat was fixed with stereotactic equipient and a burr-hole was made at 0,8mm lateral, 1.6mm posterior to Bregma. 30 gaze needle was inserted to the brain through the burr hole to reach 3.7mm depth. Affter that, 30μL of 30% kaoline solution with 60 minute sonication was injected slowly. Hydrocephalus could be inducet at 5 weeks after operation. While conventional hydrocephalus model induced by intra cisterna magna injection of kaolin died around 2 months after surgery, our model could survive much longer. Moris water maize test and Beams test revealed our iNPH modell rat showed significantl learning and memory distuubande and gait disturbande.
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Free Research Field |
脳神経外科
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Academic Significance and Societal Importance of the Research Achievements |
高齢化社会を迎え、treatable dimentiaであるiNPHの病態解明は、豊かな高齢者社会のために重要と考えられる。本研究は、iNPHを的確にmimickする水頭症モデルが開発されたことにより、今後の病態解明につながると考えられ。極めて意義が高い研究と言える。
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