Development of novel spray-type fluorescent probes as adjunct to surgery for malignant brain tumors

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K09365
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56010:Neurosurgery-related
• Research Institution: Okayama University (2023); The University of Tokyo (2020)
• Principal Investigator: Tanaka Shota 岡山大学, 医歯薬学域, 教授 (80643725)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 脳腫瘍 / 蛍光プローブ / 低悪性度神経膠腫 / 中枢神経系原発悪性リンパ腫 / 転移性脳腫瘍

Outline of Final Research Achievements

Fluorescence labeling is a surgical adjunct in brain tumor surgery useful in visualizing tumor to achieve maximal safe resection. We previously developed a novel fluorescent probe for glioblastoma; it is applied topically unlike oral 5-aminolevulinic acid (5-ALA), the approved probe for malignant glioma surgery. In this study, we have developed a fluorescent probe efficacious for low-grade glioma which is undetectable by 5-ALA. In addition, we have developed fluorescent probes efficacious for primary central nervous system lymphoma. Fluorescence is also ascertained in a patient-derived orthotopic xenograft in a mouse model. As for metastatic brain tumors, it is suggested that the fluorescent probe known to be efficacious for the primary tumor tends to good for the metastatic tumor.

Free Research Field

脳腫瘍

Academic Significance and Societal Importance of the Research Achievements

低悪性度神経膠腫は、膠芽腫と異なり周囲脳組織との判別が困難であり、5-ALAも無効であるため、有効な蛍光プローブが開発されれば、低悪性度神経膠腫の手術を一変させ得る革新的新規技術となる。中枢神経系原発悪性リンパ腫では、後療法が極めて有効なため外科手術は生検に留める。術中診断は手術の有効性のリアルタイムな評価のため重要で、有効な蛍光プローブが開発されれば、術中迅速蛍光診断が可能となり、病理診断体制が充分でない施設では特に有用な技術となる。転移性脳腫瘍では、外科手術での全摘出が求められるため、有効な蛍光プローブが存在すれば術中に蛍光診断での確認が可能となり、意義が大きい。