Development of monitoring system for rapid detection of orthopaedic SSI using the portable DNA sequencer MinION

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K09419
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: Kansai Medical University
• Principal Investigator: Kushida Taketoshi 関西医科大学, 医学部, 非常勤講師 (70411527)
• Co-Investigator(Kenkyū-buntansha): 松尾 禎之 関西医科大学, 医学部, 講師 (50447926)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 次世代シーケンサー / MinION / インプラント感染

Outline of Final Research Achievements

The authors planned to collect specimens from patients in the early postoperative period after cervical spine surgery, perform sequencing, and verify the effectiveness of MinION in detecting early postoperative infection with the goal of establishing a rapid identification method for bacteria using MinION and bringing about a major change in the diagnosis of infectious diseases. However, due to the decrease in the number of surgical cases caused by the spread of new coronary infections and the decrease in the occurrence of implant infections caused by minimally invasive spine surgery, which is a feature of our surgical method, it was difficult to secure the target patients and accumulate data on the infected cases.
Therefore, we were unable to establish a method for early diagnosis of perioperative implant infections using MinION.

Free Research Field

整形外科

Academic Significance and Societal Importance of the Research Achievements

今回、インプラント感染症例がほとんど発生しなかったことによりMinIONを用いた周術期インプラント感染の早期診断法は確立できなかったが、当院で行われている低侵襲脊椎固定手術手技はインプラント感染の予防に大きく貢献していることが明らかであり、80歳以上の高齢者、糖尿病、アレルギー性皮膚炎、抗リウマチ薬、免疫抑制薬の内服などの免疫機能低下を来す併存症を有する患者には非常に有用であることが本研究が成立しなかった点からも実証されたと言える。