Bone regeneration with combination of osteogenic matrix cell sheet and induced membrane technique

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K09438
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: Nara Medical University
• Principal Investigator: Kawamura Kenji 奈良県立医科大学, 医学部, 准教授 (20445076)
• Co-Investigator(Kenkyū-buntansha): 清水 隆昌 奈良県立医科大学, 医学部, 学内講師 (70464667) ; 面川 庄平 奈良県立医科大学, 医学部, 教授 (70597103)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 骨再生 / 骨欠損 / 細胞シート

Outline of Final Research Achievements

Bone regeneration of the rat femoral bone defects by osteogenic matrix cell sheet combined with induced membrane technique was investigated. The 10 mm bone defect was made at rat femoral bone and fixed with a locking plate. Poly Methyl Methacrylate（PMMA）was filled in the bone defect at the first surgery. Osteogenic matrix cell sheet was made from cultured mesenchymal stem cells. The osteogenic matrix cell sheet was implanted around the PMMA. At the second surgery, the βTCP was implanted in the bone defect after removal of the PMMA. Compared to the non-transplanted group, the osteogenic matrix cell sheet transplantation group showed better bone regeneration over time by x-ray. At 12 weeks after surgery, none of the osteogenic matrix cell sheet transplantation group developed pseudoarthrosis, whereas some individuals in the non-transplanted group developed pseudoarthrosis.

Free Research Field

整形外科

Academic Significance and Societal Importance of the Research Achievements

骨欠損の新たな再建法として臨床で注目されているInduced membrane techniqueは、高度な技術を必要としない利点があるが、大量の自家海綿骨移植を必要とするのが欠点である。Induced membrane（生体内誘導骨形成膜）に骨形成細胞シートを付加することで、骨再生能力を増強出来れば、移植に必要な海綿骨を減量させるが可能となる。本法がよりドナー障害の少ない骨欠損の再建法と成り得ることが動物実験で示唆された。