Investigation of the efficacy of direct intra-articular administration of CD146/271-positive adipose stem cells in the treatment of osteoarthritis.

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K09498
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: University of Fukui
• Principal Investigator: Miyazaki Tsuyoshi 福井大学, 福井大学・学術研究院医学系部門(附属 病院部), 客員准教授 (80324169)
• Co-Investigator(Kenkyū-buntansha): 内田 泰善 福井大学, 学術研究院医学系部門(附属病院部), 医員 (60838704)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 変性軟骨 / 間葉系幹細胞 / 関節内直接投与 / CD271

Outline of Final Research Achievements

We evaluated the effect of direct intra-articular administration of CD271-positive MSCs on degenerative cartilage regeneration in an animal model of OA. 10-week-old nude rats were treated with MIA in the knee joint to create an OA animal model, and AD-MSCs isolated from human adipocytes were used as administered cells. Cells expressing the surface antigen CD271 were isolated and designated as CD271+ MSCs, and cells containing all of them before isolation were designated as CD271-MSCs. The gross cartilage degeneration score was significantly lower in the CD271+ group. The percentage of cells positive for pain-related substances in the DRG was significantly lower in the CD271+ group. On the other hand, TNF-α and IL6 measurements in the knee synovium were similar in the CD271- and CD271+ groups.

Free Research Field

変形性膝関節症

Academic Significance and Societal Importance of the Research Achievements

今回我々はMIAを用いた軟骨変性動物モデルに対して、CD271+ MSCsを関節内に投与することで、CD271-MSCsと比較してより高い軟骨保護効果を認めただけでなく、DRGでのCGRP、substance Pの発現低下という、高い除痛効果を示す結果であった。今後、早期変性軟骨に対してこれまでDisease modifying drugが存在していない中、間葉系幹細胞の直接投与による変性軟骨再生への道が開けるものと考えられる。