2022 Fiscal Year Final Research Report
Establishment of therapeutic strategy for corneal stromal scaring treatment by applying the NF1 (von Recklinghausen disease) gene.
Project/Area Number |
20K09791
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 56060:Ophthalmology-related
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Research Institution | Ehime University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
林 康人 愛媛大学, 医学部, 研究員 (70314953)
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | 角膜実質 / ケラトサイト / レックリングハウゼン病 / バイオイメージング / 透明治癒 |
Outline of Final Research Achievements |
Corneal opacities, impar visual acuity and quality of life, were caused by prolonged inflammation and irregular proliferation of cornea stromal cells after epithelial defect due to intraocular surgery or corneal injuries. On the other hand, neurofibromatosis type I (NF-1), or von Recklinghausen syndrome, is a complex multi-system human disorder caused by the mutation of neurofibromin. Tissue specific neurofibromin knockout mice were made for studying the molecular mechanism of corneal clarity maintenance. Severe cornea stromal opacities were observed after epithelial defect in some of keratocyte inducible neurofibromin knockout. In the stromal opacity, ex-vivo confocal microscopy observation revealed that neutrophil leukocyte migration and transformation of keratocytes to myofibroblasts. Others of keratocyte inducible neurofibromin knockout corneas showed same clarity as non-induced control corneas. Unknown factors should be considered in this mouse models.
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Free Research Field |
角膜
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Academic Significance and Societal Importance of the Research Achievements |
角膜実質混濁は高度の視機能低下を招き、生活の質を低下させ、就業の選択範囲を狭める。実質混濁の機序は炎症反応の遷延、実質細胞(ケラトサイト)の不規則な増生と形態異常など様々である。角膜感染や角膜外傷後、角膜を透明で滑らかなドーム状の形状を取り戻すための治療は視力回復のためには不可欠であるが、現時点では、強い混濁を残した瘢痕治癒をする患者が一定数存在する。神経線維腫症I型患者の角膜では上皮欠損が遷延すると、実質の強い混濁が起きることを経験しており、適切な治療のためには、病態の解明が必要である。今回、そのモデルマウスが作製できたことは、今後の治療法解明に1歩前進したと考えている。
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